| Summary: | 碩士 === 國立臺灣海洋大學 === 食品科學系 === 93 === Abstract
Allergy owing to inappropriate immune responses to antigen in the environment. Asthma, allergic rhinitis and atopic dermatitis are common diseases which all belong to Type I allergic responses (so called IgE-mediated allergic responses). In recent study, it shows higher relationship between ROS and allergic responses. Curcumin is a major yellow pigment isolated from the spice turmeric. Dietary curcumin possesses an anti-oxidant and anti-inflammatory properties and it also exhibits significant chemopreventive activity. Rat basophilic leukemia cells (RBL -2H3) express α, β and γ chain of Fc�悐I, which plays a major role in the IgE-mediated allergic response. Antigen-IgE crosslinking initiates the release of ��-hexosaminidase, which is a marker of degranulation in RBL-2H3. Curcumin shows activity against ��- hexosaminidase release from IgE-mediated at 5 �嵱, even effective in calcium ionophores (A23187)-induced degranulation at��10 �嵱. Furthermore, effects of curcumin on antigen/A23187 induced release of histamine were also examined, both of them were decreased accompanied with the increasing concentration of curcumin. Among IgE-mediated allergic responses, antigen induces mast cells intracellular ROS production significantly. Curcumin could reduce ROS production from IgE-mediated or A23187 induced at 5 �嵱, and inhibits PKC-�� translocation from cytosolic to particulate.
Fc�悐I consists of four subunits (������2), and the �� chain is high affinity to IgE. The surface expression of Fc�悐I in human basophilic cell line (KU812) also studied, treating with dose-dependent curcumin expresses lower level of mRNA expression of Fc�悐I ���nand ���nchain. Even 10 �嵱 curcumin could also suppress IL-4/IL-13 mRNA expression. And all above, these results suggest that curcumin can alleviate the IgE-mediate allergic responses through down-regulating Fc�悐I expression and reducing the production of the allergic mediates and ROS.
Key words: Curcumin, IgE-mediated allergic responses, degranulation, ROS, PKC-���z Fc�悐I
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