| Summary: | 碩士 === 國立臺灣海洋大學 === 食品科學系 === 93 === Abstract
Plant sterol not only can decrease cholesterol absorption and thus protect against atherosclerosis but also have a beneficial effect against cancer. In addition, steryl glycosides have immunomodulating activities. Total plant sterol contents were usually determined in different plant materials which were the total amounts of free sterols (FS), steryl esters (SE), steryl glycosides (SG), and acylated steryl glycosides (ASG). Recently, reliable data on concentrations of phytosterol species in various plant-based foods were highly desired. Sterols in more complex sample were determined by using a method that comprised of acid hydrolysis and alkaline hydrolysis to provide the amount of total sterols. SG were determined by the deduction the total sterols contents by the sterol contents resulted by acid hydrolysis treatment. In this study, sitosterol-��-glucoside in different plants (Dioscorea pseudojaponica, and marine algae, such as Spirulina spp., Ulva lactuca, Gracilaria coronopifolia and Gloiopeltis tenax) were analyzed by RP-HPLC. Quantification of sitosterol-��-glucoside by RP-HPLC analysis was higher than using traditional method. Although the retention times of sitosterol-��-glucoside of some materials were closed to the authentic standard, it was unlikely that the signal represented to sitosterol-��-glucoside after a closer examination of their UV spectra.
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