Investigation of function of heme oxygenase in dendritic cell and CDK inhibitor, olomoucine, reduce LPS – mediated maturation of dendritic cell

• Main Authors: Tz -I Lin, 林子奕
• Other Authors: Shye-Jye Tang
• Format: Others
• Language: zh-TW
• Published: 2005
• Online Access: http://ndltd.ncl.edu.tw/handle/28181829020546069698

碩士 === 國立臺灣海洋大學 === 生物科技研究所 === 93 === Dendritic cells ( DC ) are potent antigen-presenting cells ( APC ) that play a key role in regulation of adaptive immune system. The cells are capable of presenting antigen to promote immune response. Heme oxygenase involves the metabolism of heme. This enzyme has shown that it modulates the allo-immune response by promoting activation-induced cell death of T cells. In this study, DC treated with HO inhibitor were observed the change on mix-lymphocyte reaction. In addition, the survive rate of dendritic cell was increased by the treatment of HO inhibitor during maturation. DC was induced the maturation by using LPS. In previous study, olomoucine, cyclin-dependent kinase ( CDK ) inhibitor, may inhibit the pro-inflammatory response by reducing the activation of JNK and NF-κB. In this study, olomoucine might cause cell death during maturation, reduce the NO production, and decrease the expression of CD11c as well as CD40 after LPS stimulation. Our results provides a novel finding that olomoucine may affect LPS-mediated maturation of dendritic cell.