Utilization of Fluorescent Silica Nanotubes as DNA Hybridization Bioreactors and Their Analysis

• Main Authors: Yi-Yun Tsai, 蔡依芸
• Other Authors: Chia-Chun Chen
• Format: Others
• Language: zh-TW
• Published: 2005
• Online Access: http://ndltd.ncl.edu.tw/handle/10191616505120466834

碩士 === 國立臺灣師範大學 === 化學系 === 93 === In this paper, we utilized silica nanotubes mimicing biochips to detect diseases. Silica nanotubes were prepared by a sol-gel reaction using the anodic aluminum oxide membrane (AAO) as a template. By utilizing template methods, a large number of hollow structure nanotubes were synthesized and their sizes were controlled precisely. The silica nanotubes have a number of advantages that make them potential candidates for biological applications. They have inner voids that can be immobilized with biomolecules. Also, they have distinct inner and outer surfaces that can be functionalized differentially. Hence, nanotubes can be used as smart nanophase extractors. In our experiment, the fluorescent silica nanotubes were synthesized by dopping water-soluble nanocrystal CdSe(ZnS). They were then coupled to fluorescent DNA to observe fluorescence change. Finally, we found that our nanotubes have high sensitivity in biochip analysis. So, we combined QDs-tagged silica nanotubes with fluorescent DNA as analytical tools to detect cancer disease.