Determination of corticosterone and 17-hydroxycorticosterone in plasma and urine samples by sweeping techniques using micellar electrokinetic chromatography

• Main Authors: Mei-Chuan Chen, 陳美娟
• Other Authors: Cheng-Huang Lin
• Format: Others
• Language: zh-TW
• Online Access: http://ndltd.ncl.edu.tw/handle/93400790515911433346

碩士 === 國立臺灣師範大學 === 化學系在職進修碩士學位班 === 93 === The analysis of corticosterone in mouse blood serum (metabolic-stress experiment) and 17-hydroxycorticosterone in human urine (exercise-stress experiment) samples by means of capillary electrophoresis/UV absorbance in conjunction with online sample concentration techniques is described. The use of normal MEKC had an analyte detection limit of 7 μg/mL (S/N =3); whereas when online sample concentration methods, including sweeping-micellar electrokinetic chromatography (sweeping-MEKC) and cation-selective exhaustive injection-sweep-micellar electrokinetic chromatography (CSEI-sweep-MEKC) were used, the detection limits could be improved to 3 and 5 ng/mL, respectively. In the analysis of actual samples from animal metabolic-stress experiments (39 mouse), chronically stressed animals showed a higher level (552 ± 152 ng/mL) and acute stressed animals showed an intermediate level (375 ± 105 ng/mL). In comparison, normal animals show a lower concentration level of corticosterone (153 ± 109 ng/mL). In addition, based on a human exercise-stress experiment (seven volunteers), the acute stressed humans (after exercise, 800m of running) show a higher concentration of 17-hydroxycorticosterone (137 ± 55 ng/mL for males; 128 ± 25 ng/mL for females) and the non-stressed humans (before exercise) show a lower concentration (63 ± 37 ng/mL for male; 60 ± 20 ng/mL for female), respectively. Furthermore, this method requires only small amounts of plasma and could be used as a routine tool in pharmacological studies dealing with different types of drugs, whether derived from natural herbs or by synthesis.