Studies in Vitro Fiber Digestibility of Coprophilous Fungi on Three Kinds Domestic Forage

• Main Authors: Chih-Hao Chang, 張志豪
• Other Authors: Pau-Ju Lee
• Format: Others
• Language: zh-TW
• Published: 2005
• Online Access: http://ndltd.ncl.edu.tw/handle/81079426339645800944

碩士 === 國立臺南大學 === 自然科學教育學系碩士班 === 93 === The aim of the study was to characterize the degradation activity of coprophilous fungi on three types domestic forage samples. Growth curves of nine fungi on nilegrass No1. (Acroceras macrum Stapf AC15), napiergrass taishigrass No.2 (Pennisetum purpureum Schum A146 × Pennisetum purpureum Schum A149) and pangolagrass (Digitaria decumbens Stent A254) forage are demonstrated. After initial experiments for testing the potential of cellulase of coprophilous fungi were carried out by the interaction of the directed Congo red on cellulose based Mandels-Reese agar at different pH, Circinella mucoroides, Ascodesmis nigricans, Ascobolus scatigenus, Coprinus patouillardii and Coprinus stercoreus were selected to estimate the digested effect on forage fiber by series detergent methods including neutral and acid detergent fiber (ADF, NDF) and ash content based on the phases of fungal growth pattern. The digestion of cellulose, hemicellulose and lignin were computed by variations among NDF, ADF and ash digestion values. Results demonstrated that the variations and relationship patterns between NDF, ADF and ash varied from sample to sample. While computed on series digestion values, presumably conclusions showed that C. patouillardii and C. stercoreus carry the digestion ability of cellulose, hemicellulose and lignin on nilegrass No1. forage; A. scatigenus and C. stercoreus on napiergrass taishigrass No.2 forage. The usages of soluble CHO for growth of Ascobolus scatigenus were higher than C. mucoroides and A. nigricans on nilegrass No1. forage. C. mucoroides only uses soluble CHO on napiergrass taishigrass No.2 forage. The highest ash content was in degradation process of C. mucoroides and C. patouillardii. However, the ash contents of dry matter can be an influenced factor in the determination of fiber digestion.