Construction of Dynamic Gene Regulatory Network of Hela Cell Cycle via Microarray Data

• Main Authors: Yung-Hsiang Chu, 朱永翔
• Other Authors: Bor-Sen Chen
• Format: Others
• Language: zh-TW
• Published: 2005
• Online Access: http://ndltd.ncl.edu.tw/handle/01848787866403583567

碩士 === 國立清華大學 === 電機工程學系 === 93 === Cell cycle is an important clue to unravel the mechanism of cancer cells. Therefore, it is important topic to study the gene regulatory network of cancer cell cycle. Recently, expression profiles of cDNA microarray data of cancer cell cycle are available. Therefore, it is more appealing to construct a dynamic model for gene regulatory network of cancer cell cycle to gain more insight into the infrastructure of gene regulatory mechanism of cancer cell via microarray data. Then, based on the dynamic model, we will discuss the regulatory abilities of regulatory genes to find their roles in the network. A dynamic model is proposed for the gene regulatory network of cancer cell cycle. Based on the gene regulatory dynamic model and the cDNA expression profiles of the target gene, the regulatory function from upstream regulators is detected by a correlation algorithm to trace back their upstream regulatory genes.　 Then we can construct the regulatory network between target genes and their upstream regulatory genes by identifying the chemical kinetic parameters of regulatory pathways via the cDNA expression profiles of the target genes and their upstream regulatory genes. Then these upstream regulatory genes are considered as target genes. 　We trace back their upstream regulatory genes. Iteratively, we will construct the whole dynamic gene regulatory network of cancer cell cycle which is useful to gain more insight into the whole mechanism of cancer cell. Finally, based on the dynamic regulatory network, we analyze the regulatory abilities of regulatory genes to discuss their roles in the network of cancer cell cycle.