| Summary: | 碩士 === 國立清華大學 === 生物資訊與結構生物研究所 === 93 === Phalaenopsis orchids, including cultivars of Phalaenopsis and its inter-generic hybrid with Doritis and Doritaenopsis, are commercially important plants cultivated as cut flowers and pot plants around the world due to their flamboyant, vivid colours and their dramatic shapes, with wide variations generated through inter-specific and inter-generic hybridization.
Flavonoid-3’,5’-hydroxylase (F3’5’H), a member of the cytochrome P450 family, is the key enzyme in the synthesis of 3’,5’-hydroxylated anthocyanins, which are generally required for blue or purple colors of flowers. Phalaenopsis orchids lack F3’,5’H activity and are, therefore, unable to generate purple or blue flowers. However, in the 90s, “black flower” hybrids series with distinct fuchsia spots or dark purple colored blooms are available in market. In this study, the RT-PCR fragments of F3’,5’H, where prepared from isolated RNA of several these “black flower” hybrids petal . The deduced sequence of the F3’,5’H fragments have close to 100% identity to that of petunia. Furthermore, the petunia difF encoding a cytochrome b5, Cauliflower mosaic virus(CaMV) 35S and Nopaline synthase(NOS) mRNA polyadenylation signal sequences where also detected in these “black flower” hybrids genomic background via PCR approach . These data provide evidences suggesting that some “black flower” hybrids now available in market maybe been not hybrids based on conventional sexual crossing but genetically modified organisms (GMO) crops .
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