Analysis of Tilapia, Oreochromis mossambicus, Serotonin Receptor Promoter

• Main Authors: Tsai-jia shiun, 蔡佳訓
• Other Authors: Ching-Lin Tasi
• Format: Others
• Language: zh-TW
• Published: 2005
• Online Access: http://ndltd.ncl.edu.tw/handle/75555757833376294519

碩士 === 國立中山大學 === 海洋資源學系研究所 === 93 === The central 5-hydroxytryptamine ( Serotonin, 5-HT ) system play an important role in the brain sexual differentiation in tilapia,Oreochromis mossambicus. 　The 5-HT1A receptor is involved in the neural develop- ment of brain.　The cDNA sequences of 5-HT1A receptor of tilapia, Ore- ochromis mossambicus have been cloned in our laboratory.　In the present study, 1633 bp of DNA sequence of 5-HT1A receptor from the transcription start site ( TSS ) were cloned by 5’ rapid amplification of cDNA end ( 5’ RACE ) and Genome Walker DNA Walking.　The 1633 bp of DNA sequence divided into six different fragments to detect the luciferase activity in the HeLa cell.　The bioinformatic analysis was performed on the fragment which showed the highest luciferase activity for predicting the transcription factor binding site.　These results showed that there is a activator protein-1 ( AP-1 ) binding site in the fragment form -52 bp to -46 bp.　The site-direct mutagenesis and electrophoresis mobility shift assay was performed on the fragment form -52 bp to -46 bp .　The present results indicate that there is a transcription factor binding site in the fragment form -52 bp to -46 bp.　This transcription factor binding site could be a AP-1 binding site.