Development of Chicken Infectious Anemia Inactivated Vaccine
碩士 === 國立屏東科技大學 === 獸醫學系 === 93 === One Taiwan field isolate of chicken infectious anemia virus ( CIAV ) high virulence and high growth titer in MDCC-MSB1 cells was chose for figuring out of its most suitable multiplicity of infection in order to propagated the most high yield ( 109.2 TCID50/mL ) of...
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ndltd-TW-093NPUST5410102016-12-22T04:11:09Z http://ndltd.ncl.edu.tw/handle/54368133764033082347 Development of Chicken Infectious Anemia Inactivated Vaccine 雞傳染性貧血不活化疫苗之研發 Li-Shiang Hong 洪麗香 碩士 國立屏東科技大學 獸醫學系 93 One Taiwan field isolate of chicken infectious anemia virus ( CIAV ) high virulence and high growth titer in MDCC-MSB1 cells was chose for figuring out of its most suitable multiplicity of infection in order to propagated the most high yield ( 109.2 TCID50/mL ) of CIAV virus suspension by suspension cultivation. The CIAV supernatant was then binary ethylene imide inactivated and prepared as gel or oil-emulsion type inactivated vaccine for 2 times immunization in a group 3-week-old SPF chickens, 20 birds per group, a live CIAV vaccine and control group was also included. Five birds per group were evaluated for hematocrit value ( HV ) at 10 and 21 day post immunization ( DPI ) and 10 birds was evaluated enzyme- linked immunosorbent assay ( ELISA ) at 28 DPI. All the experimental chickens were found with normal HV ( >25% ) except all the control and live vaccine immunized groups ( 3 and 2 in Trial 1 and 2 respectively ). The ELISA titer in Trial 1 was found highest in the gel vaccine immunized group ( 5612 ), then the live ( 5120 ) and OEV ( 1441 ) immunized group. The ELISA titer in Trial 2 was found highest in the OEV vaccine ( 6861 ) due to changing the oil- adjuvant, then the gel type ( 5056 ) and live ( 4274 ) vaccine. The Taiwan field isolated CIAV propagated in MDCC-MSB1 cell by suspension cultivation provide good potential in the preparation of high immunoprotectivity of inactivated CIAV vaccine. Maw-Yeong Lin 林茂勇 2005 學位論文 ; thesis 68 zh-TW |
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碩士 === 國立屏東科技大學 === 獸醫學系 === 93 === One Taiwan field isolate of chicken infectious anemia virus ( CIAV ) high virulence and high growth titer in MDCC-MSB1 cells was chose for figuring out of its most suitable multiplicity of infection in order to propagated the most high yield ( 109.2 TCID50/mL ) of CIAV virus suspension by suspension cultivation. The CIAV supernatant was then binary ethylene imide inactivated and prepared as gel or oil-emulsion type inactivated vaccine for 2 times immunization in a group 3-week-old SPF chickens, 20 birds per group, a live CIAV vaccine and control group was also included. Five birds per group were evaluated for hematocrit value ( HV ) at 10 and 21 day post immunization ( DPI ) and 10 birds was evaluated enzyme- linked immunosorbent assay ( ELISA ) at 28 DPI. All the experimental chickens were found with normal HV ( >25% ) except all the control and live vaccine immunized groups ( 3 and 2 in Trial 1 and 2 respectively ). The ELISA titer in Trial 1 was found highest in the gel vaccine immunized group ( 5612 ), then the live ( 5120 ) and OEV ( 1441 ) immunized group. The ELISA titer in Trial 2 was found highest in the OEV vaccine ( 6861 ) due to changing the oil-
adjuvant, then the gel type ( 5056 ) and live ( 4274 ) vaccine. The Taiwan field isolated CIAV propagated in MDCC-MSB1 cell by suspension cultivation provide good potential in the preparation of high immunoprotectivity of inactivated CIAV vaccine.
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author2 |
Maw-Yeong Lin |
author_facet |
Maw-Yeong Lin Li-Shiang Hong 洪麗香 |
author |
Li-Shiang Hong 洪麗香 |
spellingShingle |
Li-Shiang Hong 洪麗香 Development of Chicken Infectious Anemia Inactivated Vaccine |
author_sort |
Li-Shiang Hong |
title |
Development of Chicken Infectious Anemia Inactivated Vaccine |
title_short |
Development of Chicken Infectious Anemia Inactivated Vaccine |
title_full |
Development of Chicken Infectious Anemia Inactivated Vaccine |
title_fullStr |
Development of Chicken Infectious Anemia Inactivated Vaccine |
title_full_unstemmed |
Development of Chicken Infectious Anemia Inactivated Vaccine |
title_sort |
development of chicken infectious anemia inactivated vaccine |
publishDate |
2005 |
url |
http://ndltd.ncl.edu.tw/handle/54368133764033082347 |
work_keys_str_mv |
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