The Establishment and Comparison of Tissue Culture System of Three Solanaceous Plants（Capsicum annuum L.、Lycopersicon esculentum Mill.、Solanum melongena L.）

• Main Authors: Shou-Yin Tai, 戴守音
• Other Authors: Li-Jen Liao
• Format: Others
• Language: zh-TW
• Published: 2005
• Online Access: http://ndltd.ncl.edu.tw/handle/45170028190139217820

碩士 === 國立高雄師範大學 === 生物科學研究所 === 93 === This study was to investigate the callus induction and bud regeneration of three solanaceous plants by using stem segments. To reach the purpose of this study, we composited MS medium with various concentrations of plant growth regulators to determine the efficiency of callus induction and bud regeneration. The results as summarized as follows: 1. Callus formation in Capsicum annuum L. can be induced by MS medium supplemented with 10 ppm NAA. The callus proliferation can get in 72% after 14 days. 2. Callus formation in Lycopersicon esculentum Mill. can be induced by MS medium supplemented with 1ppm NAA+ 1 ppm kinetin. Than the callus transplanted to MS medium supplemented with 1.69~2.82 ppm BA could induce shoot formation. It is most helpful to induce root formation when MS medium was supplemented with Hyponex No.4 0.01%-0.05%. 3. Callus formation in Solanum melongena L. can be induced by MS medium supplemented with 0.1ppm NAA. Than the callus transplanted to MS medium supplemented with 0.93 ppm NAA could induce shoot formation. It is most helpful to induce root formation when MS medium was supplemented with Hyponex No.4 0.01%-0.05%. In this study, the plantlets regeneration can be induced in Lycopersicon esculentum Mill. and Solanum melongena L.. But the Capsicum annuum L. could only develop to the callus formation without bud regeneration, so it need to work hard in the aspects of their tissue culture.