On-line solid-phase extraction used C4 coupled with high performance liquid chromatography for the quantitative determination of fourteen sulfonamides in milk

• Main Authors: CHANG,WEN-HSING, 張文興
• Other Authors: 楊慶成
• Format: Others
• Language: zh-TW
• Published: 2005
• Online Access: http://ndltd.ncl.edu.tw/handle/29246469724399888064

碩士 === 國立高雄師範大學 === 化學系 === 93 === Abstract This study describes a newly developed solid-phase extraction (SPE) used C4 on-line with high performance liquid chromatography (HPLC) method for the quantitative determination of fourteen commonly used sulfonamide residues in milk. Factors affecting analysis such as separation temperature, pH and the injection volume of sample solution were investigated to obtain the optimum conditions. By the optimum conditions, the linear range, reproduction and the detection limit of the analysis method will be measured, then apply the developed method to analyze sulfonamides of commercial milk. The on-line SPE is proposed to be a new pre-treatment technique having advantages that to avoid losing samples and it is fast and easy to use. Experimental results indicated that fourteen sulfonamides are well separated in an acceptable time on the reversed-phase C18 column eluted with the gradient elution of formic acid aqueous solution/acetonitrile at 1.0 ml/min flow-rate and to set the detection wavelength at 270nm. The optimal conditions of the on-line SPE analysis are 800μl of injection volume and the sample solution adjusted to pH 5. Analyzing sulfonamides of three kinds of milk by using the new technique, the recoveries ranging from 79 to 119% of fourteen sulfonamides were determined. The study results showed that the SPE used C4 on-line with HPLC method has the applicability to the analysis of sulfonamides in milk.