| Summary: | 碩士 === 國防醫學院 === 生物及解剖學研究所 === 93 === In this study, we used anti-protein kinase C ε antibodies to investigate the distribution and expression of the PKC epsilon(PKCε)in neurosphere and the role of PKCε in neurogenesis. Neural precursor cells could self-renew and have the potential to differentiate into neurons or glia, indicating that they have characteristics of neural stem cells. In serum-free defined medium supplemented with epidermal growth factor(EGF), suspension cells dissociated from the postnatal rat brain cortex had ability to proliferate and form floating spherical colonies, called neurospheres. After replacement with defined medium contained fetal bovine serum, the neurospheres could attach, migrate and differentiate into neurons, astrocytes and oligodendrocytes. PKCε staining showed that PKCε was expressed abundantly in neurosphere. Double immunofluorescence showed that cells migrated out of the neurosphere differentiated into neural precursor, astrocyte, oligodendrocyte and neuron, and PKCε expressed in neuron and immature oligodendrocyte. Western blotting analysis confirmed the phenomenon by immunofluorescence that cells migrated out of the neurosphere had reduced PKCε expression gradually with increasing incubation time. Furthermore, the active form of PKCε had maximum expression in early differentiation, but downregulation after 1 day subculture. These data suggest that PKCε may involved in neurogenesis.
|
|---|
