| Summary: | 碩士 === 國立嘉義大學 === 農業生物技術研究所碩士班 === 93 === Fowlpox virus (FPV) has long been identified as the species of family Poxviridae and characterized to have a genome size of about 288 kilo base-pairs (kb) with a double stranded and linear DNA. This causative agent was shown to produce lesions on the skin (cutaneous form) and / or in the mouth, esophagus and trachea (diphtheritic form) of avian at all ages. Vaccines have been employed to control FPV infection in avian industry. Yet, sporadic outbreaks of FPV infection in avian can still occur even after immunization. Therefore, developing new and efficient vaccines is needed for fighting against FPV infection at present. By applying the technology of polymerase chain reaction (PCR), three primer sets were designed and used to separately amplify three specific DNA fragments containing respectively the virion envelope protein gene, membrane protein gene, and immuno-dominant virion protein gene in the vaccinia strain of FPV. The 3 different PCR products were further cloned, sequenced, and characterized to have the same amino acid sequences as the previously published ones. These results will help to develop DNA vaccines or subunit vaccines against FPV in the future. The PCR technology that can amplify these viral genes was also tested and proven to be capable of detecting both vaccinia strain and field isolated FPV. Furthermore, the IgG and IgY antibodies prepared from the FPV-immunized chicken’s serum and egg yolk separately were demonstrated to recognize the protein extract of FPV by dot-blotting assay. These results pave the way of developing new and efficient vaccines against FPV in the future.
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