Signaling mechanism of Galectin-1 induced 3T3 cell proliferation on chitosan membrane

• Main Authors: Ming-Hui Hu, 胡明惠
• Other Authors: Rong-Nan Huang
• Format: Others
• Language: en_US
• Published: 2005
• Online Access: http://ndltd.ncl.edu.tw/handle/82575759779526992152

碩士 === 國立中央大學 === 生命科學研究所 === 93 === Chitosan is considered to be a very promising biopolymer for various biomedical and pharmaceutical uses because of its nontoxic and biocompatible natures. In clinical medicine, chitosan membrane has been used as a biological dressing. Galectin-1 (GAL1), a β-galactoside-binding protein, functions in cell adhesion, development, and growth regulation. Previous study in this laboratory have shown that GAL1 support 3T3 proliferation on chitosan membrane. In this study, we further investigated the mechanism of GAL1-induced 3T3 cell proliferation on chitosan membrane. The results show that GAL1 play an important role in enhancing 3T3 cell adhesion and proliferation on the surface of chitosan membrane. To better characterize a plausible signaling pathway, the 3T3 cells were pre-treated with various kinase or phosphatase inhibitors and cultured in a GAL1-coated chitosan membrane. The results show that Na3VO4 (tyrosine phosphatase inhibitor) can synergistically enhance GAL1-induced 3T3 cell proliferation on chitosan membrane. In contrast,genistein (tyrosine kinase inhibitor) and Na3VO4 had no dramatic effect on the 3T3 seeded on culture plates. Moreover, immunoprecipitation assay indicates that cdc2 kinase activity is significantly enhanced in 3T3 cells growing on GAL1-coated culture plates. The current results also suggest that GAL1 induces 3T3 cells proliferation through the ERK1/2 signal pathway. Altogether, we proposed that the signal mechanism of GAL1-induced 3T3 proliferation on chitosan membrane was mediated through ERK1/2 and cdc2 kinase.