Development of a Novel Patch-Clamp Chip by Electrowetting-on-Dielectric and Insulator-Based Dieletrophoresis

• Main Authors: Tzu-Yao Su, 蘇子耀
• Other Authors: Shih-Kang Fan
• Format: Others
• Language: zh-TW
• Published: 2005
• Online Access: http://ndltd.ncl.edu.tw/handle/48602966823224110915

碩士 === 國立交通大學 === 奈米科技研究所 === 93 === The patch clamp technique is a fundamental technique in modern electrophysiology. It can record electrical currents of single ion channel by using a tiny glass pipette to clamp native biomembrane and membrane protein. Recently this technique has been integrated into microfabricated devices, called “patch clamp chips”. Requiring no operators, patch clamp chips provide automatic and precise manipulation. In this research, we propose a novel patch clamp chip by using electrowetting-on-dielectric (EWOD) and insulator-based dielectrophoresis (iDEP) techniques to manipulate bio-fluids, bio-objects, and liposome. EWOD has been successfully used to transport, cut, and merge potassium chloride droplets in single-sided electrode devices. 2D and 3D iDEP have been demonstrated to trap DNA molecules. Microcontact printing and electroformation were investigated to prepare monodispersed giant liposomes, although not very successfully. We used painted methods to form artificial lipid bilayer and observed it under a microscope. Several basic techniques were examined in this research to fulfill an automatic patch clamp chip for membrane protein study. We hope to demonstrate a high-throughput drug screening microdevice in the future on the basis of integration of the techniques investigated in this research.