Study the Applications of Antigen-Antibody Related Reactions in Analytical Magnetapheresis

• Main Authors: Yu Ping Lin, 林玉瓶
• Other Authors: C. Bor Fuh
• Format: Others
• Language: zh-TW
• Published: 2005
• Online Access: http://ndltd.ncl.edu.tw/handle/78372089670778989094

碩士 === 國立暨南國際大學 === 應用化學系 === 93 === The thesis is divided into two parts. In Part I, analytical magnetapheresis was used to study the interaction of antigen and antibody. In Part II, we studied the dispersion of nanopowder（silica, SiO2）under different surfactants. Part I：Analytical magnetapheresis has become a useful separation technology, in recent years. It is mainly used for macro molecules（MW＞106）, colloids and particles. This technology was fast, simple, selective and nondestructive. This study used the gradient elution principle. It studied the interactions of Streptavidin-Biotin and IgG-Protein A in analytical magnetapheresis. Experimental parameters were studied under different conditions of the flow rate, injected particles and material channels. The Streptavidin-conjugated magnetic beads and IgG-conjugated magnetic beads can capture silica particles labeled with Biotin and Protein A in the separation channel under magnetic filed. The selectivity is high with controlled experiments. Part II：Nano-scale materials have some special physics properties. Nano-materials play a major role for their physics properties. Aggregation must be avoided for applications of nanopowder. We added surfactant into solution to improve the aggregation condition of silica nanopowder. We have tested several surfactants. Dispersion was improved with adding 0.01％ of FL-70 surfactant into solution.