Analysis of Inositol Isomers by Capillary Electrophoresis

• Main Authors: Jin-Yi Chen, 陳錦益
• Other Authors: 曾惠芬
• Format: Others
• Language: zh-TW
• Published: 2005
• Online Access: http://ndltd.ncl.edu.tw/handle/76318213515475103712

碩士 === 國立暨南國際大學 === 應用化學系 === 93 === Myo-inositol plays an important role in the inositol/lipid cycle, which is the source of metabolic intermediates for such processes as signal transduction, steroid synthesis, and intracellular calcium regulation. It is known to be effective in the treatment of both panic disorder and obsessively compulsive disorder, while scyllo-inositol may be a marker for cerebral pathology. In addition, they may represent a viable treatment for Alzheimer’s disease. Two capillary electrophoresis methods were developed for separation of myo-inositol and scyllo-inositol. The first system coupled capillary zone electrophoresis with the indirect UV detection. This system was simple and temperature control was not necessary. The correlation coefficients of myo-inositol and scyllo-inositol were 0.9994 and 0.9997, respectively. The concentration limits of detection of myo-inositol and scyllo-inositol were 1.44 mM and 0.98 mM, respectively. In the precision test, all relative standard deviations of migration time and area ratio were smaller than 5.1 % and 0.6 %, respectively. The second system combined micellar electrokinetic capillary chromatography with fluorescence detection N-methylisatoic anhydride was used for pre-derivatization of the non-fluorescent inositols before analyzed by MEKC. The analysis can be completed in 4 minutes and the correlation coefficients of myo-inositol and scyllo-inositol were 0.9988 and 0.9992, respectively. The concentration limit of detection was lower than 10-4 M.