Biochip fabrication of action potential measurement for single cells

• Main Authors: ji-fu lin, 林紀甫
• Other Authors: Ching-Hsing Luo
• Format: Others
• Language: zh-TW
• Published: 2005
• Online Access: http://ndltd.ncl.edu.tw/handle/49810486079887131772

碩士 === 國立成功大學 === 微機電系統工程研究所 === 93 === 　　Patch clamp is a well-developed lelectrophysiological recording technique used to study ion channel function and regulation. The conventional method of performing patch clamp technique employs a glass micropipette onto the cell by manual manipulation. Despite this technique is extremely sensitive and information-rich, but requires a highly-skilled operator and is limited in throughput. Voltage clamp that determine the behavior of the ion channel conductances responsible for the generation of the action potential is the another method to record the flow of ionic current across the cell membrance. The method is held a constant membrane potential while the ionic current flowing through the membrane is measured. 　　The thesis presents cell-platforms and etcing pores on silicon by using soft lithography and etching technique insteading of conventional glass micropipette recording method. we successfully demonstrated practicability of the process by the cells adhered on the platform and the etching pores on the silicon chip by using ICP. The less laborious manipulation、time saving and high sampling throughput will be expected .In the future, we can automatically real time controlled and changed in response to the cell’s physiologic characteristics measured by ionic channel activities.