Design, Fabrication and Control of Reverse Transcription Polymerase Chain Reaction Micro Bio-chips

• Main Authors: Chung-Yang Sue, 蘇中源
• Other Authors: Nan-Chyuan Tsai
• Format: Others
• Language: zh-TW
• Published: 2005
• Online Access: http://ndltd.ncl.edu.tw/handle/18305257072241858059

碩士 === 國立成功大學 === 機械工程學系碩博士班 === 93 === Abstract 　Being beneficial from dramatic progress in bio-chemical and micro-electro-mechanical technologies, traditional DNA manipulation devices tend to be miniaturized to speed up detection processing. Polymerase Chain Reaction (PCR) and Reverse Transcription Polymerase Chain Reaction (RT-PCR) are two typical examples of them. In this research, micro RT-PCR (μRT-PCR) chips are implemented to quantitatively detect DNA and RNA viruses. Test samples reservoirs, RT-PCR, and capillary electrophoresis are integrated on a SU-8 based monolithic chip. A high-precision temperature control system is well developed by embedding amplification circuits and an Intel 8051 microprocessor. The integrated system exhibits high throughput and adequate sensitivity to detect the infected malignant tissues in quantity by intensive simulations.