| Summary: | 碩士 === 國立成功大學 === 生物科技研究所碩博士班 === 93 === In this report, spinner flask bioreactor and BelloCell bioreactors were used to produce recombinant pro-urokinase in CHO cells. The CHO cell lines used in this study were CHO-UK and CHO-UK-Mu that producing recombinant human pro-urokinase (pro-UK) and pro-urokinase mutant (proUK-Mu), respectively. We cultivated CHO-UK cells in spinner flask bioreactor and purified the culture medium to get proUK. The SDS-PAGE of proUK showed that the CHO cells would secrete protease to cleave the single-chain proUK into two-chain UK. But we produced proUK-Mu by CHO-UK-Mu either in spinner flask or in BelloCell, the finally purified proUK-Mu was a single-chain form. It suggested the proUK-Mu would not be affected by the protease. Otherwise, we compared the yield of proUK-Mu in spinner flask and in BelloCell. The cell density and the growing environment in BelloCell were better than in spinner flask, even the productivity of a day was the BelloCell advancing.
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