upregulation of WW Domain Containing Oxidoreductase/WOX1 in the Injured Doresal Root Ganglion

• Main Authors: Meng-Yen Li, 李孟燕
• Other Authors: Jiang-Ping Wang
• Format: Others
• Language: en_US
• Published: 2005
• Online Access: http://ndltd.ncl.edu.tw/handle/94373091317596529598

碩士 === 國立成功大學 === 生命科學系碩博士班 === 93 === Abstract 　Human WWOX gene is located on a chromosomal fragile site ch16q23.3-24.1.Hight frequency of loss of heterozygosity (LOH) of this gene has been shown in multiple cancer. The WWOX gene encodes a putative tumor suppressor WW domain-containing oxidoreductase WOX1 (also known as WWOX or FOR2). WOX1 is a proapoptotic protein. Stress stimuli activate WOX1 via tyrosine 33 (Try33) phosphorylation, resulting the its complex formation with p53 and translocation to nuclear mediate apoptosis. Here, we investigate the potential role of WOX1 in L4/5 dorsal root ganglia (DRG) and spinal cord and its interaction with other transcription factors in vivo following sciatic nerve transaction. By immunocytochemistry, nuclear translocation of WOX1 was observed in DRG neurons 6 hours ipsilaterally to axotomy. WOX1 upregulation was also expressed in the cytoplasm of DRG at the contrlateral side as comparea normal control animal, but low levels in the nuclei, WOX1 expression did not change in the spinal cord. By using electron microscopy, we showed that WOX1 colocalized with immediate-early genes (IEGs) such as activation transcription factor 3 (ATF3) and c-Jun. These findings suggest that WOX1 might be involved in not only apoptosis but also the regulation of gene expression in the peripheral nerve system.