Syntheses and Properties of2,3-O-(4-Hydroxybutyl)-β-Cyclodextrin derivativesused as stationary phase for monolithic column

• Main Authors: Yat-Chi Li, 李逸祺
• Other Authors: Chun-Hsiung Kuei
• Format: Others
• Language: zh-TW
• Published: 2005
• Online Access: http://ndltd.ncl.edu.tw/handle/89312977741069100096

碩士 === 國立成功大學 === 化學系碩博士班 === 93 === Abstract 　In this study, we focus on transferring the functional groups in each glucoses-linked of β-cyclodextrin, especially on the hydroxyl groups of the C2 and C3 positions as well as the C6 terminal hydroxyl group. First, the hydroxyl group in C2 and C3 positions have used synthesized by 1-bromo-butanol to be the primary butanol species. 　On the other hand, to combine the silica particle molecule linked the primary alcohol at C6 position by using GPTMS and continued deprotecting the functional groups. All synthesized intermediate compounds were independently and conduct spectroscopy characterization including 1H-NMR and 13C-NMR. 　The terminal silica-linked β-cyclodextrin was added into the fuse capillary by high-pressure and using monolithic column technique，to prepared newly column for μ-HPLC. In addition, the prepared method by re-actively inside surface of capillary, added stationary phase and the gel combined TMOS with EtMOS for condensation reaction for 3 μm silica particles bonding at surface in capillary to be a monolithic column. In past experiences, the method of packing 3 μm silica particle molecules into prepared-column were difficult problems, and we have salve this problem, by the way , we have saw out the bubble problem when we make up a column, that will cause the broken wall in the column. 　As result, this study was developed three excellent points. The first, we can synthesized the different protect groups of β-cyclodextrin in good yields. The second, we revised the technique of drying packing column in good yields when we packing 3μm silica particle. The third, we loading the PAHs compounds to test the column, the data seems that the CD’s hole was block up, and the result was not fit in with early supposition.