Automatic Biochip Platform for Cell Separation and Nucleus Collection

• Main Authors: Jian-Shiuan Tai, 戴健軒
• Other Authors: Gwo-Bin Lee
• Format: Others
• Language: zh-TW
• Published: 2005
• Online Access: http://ndltd.ncl.edu.tw/handle/00045177872032715988

碩士 === 國立成功大學 === 工程科學系碩博士班 === 93 === 　This study reports a new biochip for cell separation and collection utilizing dielectrophoresis forces on micro-fluidic systems composing micro pneumatic pumps and valves. By using MEMS technology, micro devices could be integrated into the biochip to achieve the functions of cell transportation and sorting. With the advantages of low operation voltage, dielectrophoresis forces operated under a specific voltage and frequency could be used to manipulate cells without damaging cell samples. 　A fabrication process based on MEMS technology was developed in this study. Firstly, the dielectrophoresis electrode array was fabricated on glass substrates. Then micro flow channels, micropumps and microvalves made of PDMS (Polydimethylsiloxane) were bonded on the glass. Dielectrophoresis forces were applied on the cell samples by the electrode structures to achieve cell separation. By integrating the micro pneumatic pumps and valves, the samples with different kinds of cells could be transported in the micro channel, and moved to specific chambers after sorting by the dielectrophoresis electrode array. Experimental results showed that viable and non-viable cells (human lung cancer cell, A549) could be successfully separated and collected using the developed chip. The efficiencies of separation for the viable and non-viable cells are 84% and 81%, respectively.