A Microfluidic Chip Integrated with Molecular Imprinting Polymers for Surface Plasmon Resonance Detection

• Main Authors: Shih-Chiang Huang, 黃仕強
• Other Authors: Gwo-Bin Lee
• Format: Others
• Language: zh-TW
• Published: 2005
• Online Access: http://ndltd.ncl.edu.tw/handle/24799835502296674236

碩士 === 國立成功大學 === 工程科學系碩博士班 === 93 === 　　This study reports a novel microfluidic system integrated with localized molecular imprinting polymers (MIP) for surface plasmon resonance (SPR) detection of specific label-free bio-samples. The innovative microfluidic biochip is capable of transporting a specific amount of bio-samples inside multiple microchannels using micro pneumatic micropumps and micro pneumatic valves to sensing regions where multiple MIP films are locally spin-coated such that highly-sensitive, highly-specific bio-sensing could be achieved. 　　The innovative microfluidic biochip is fabricated by using MEMS (Micro-Electro-Mechanical System) technology on glass (BK-7) and PDMS (Polydimethylsiloxane) substrates. The flow sensor can be certain calibrated the error of pumping rate and realized the accurate sample transfer. In addition, since SPR detection could be very sensitive to temperature variation, a micromachine-based temperature control module comprised of micro-heaters and a temperature sensor was used to maintain a uniform temperature with a variation less than 0.1℃ during measurement. 　　The microfluidic MIP/SPR biochips have the potential to be widely used for bio-sensing applications. While compared to large-scale SPR techniques, the developed microfluidic system has several advantages, including labeling-free, high sensitivity, capability of quantitative analysis of nano-scale bio-molecules in real-time fashion.