The kinetic analysis of KIF1A onthe microtubule

• Main Authors: shun-yi jian, 簡順億
• Other Authors: ming-zhe huang
• Format: Others
• Language: zh-TW
• Published: 2005
• Online Access: http://ndltd.ncl.edu.tw/handle/79953070904453266553

碩士 === 國立成功大學 === 工程科學系碩博士班 === 93 === KIF1A is a one-head motor protein that moves along microtubule toward plus end. Like the other molecular motors, KIF1A use ATP hydrolysis and product release to produce movement. In this paper, we construct a model to analyze KIF1A movement, that was built up ATP hydrolyze cycle and motion behavior of KIF1A. The process is including four steps: start, detach, oscillate stochastic, and re-bind. In this model, we use the enzyme kinetics and stochastic processes analysis for computing the mean displacement and standard deviation displacement. Then, we calculate the mean displacement with external force formula. When the external force raise, the mean displacement is decreased. Finally, we adopt Langevin equation to describe the Brownian motion effect and use Forkker-planck equation to calculate probability density for stepping motion. The analysis reveals the variation of probability distribution with standard deviation displacement. The main factor in influencing standard deviation displacement is reaction time.