| Summary: | 碩士 === 國立中興大學 === 獸醫學系 === 93 === In this study, we collected 93 strains of Escherichia coli (E. coli) from May 2004 to February 2005 in middle Taiwan. These strains were tested the antimicrobial susceptibility with 14~20 antimicrobials by the Bauer Kirby Disk Diffusion Method and the Minimum Inhibitory Concentration (MIC) of 3 antimicrobials by broth dilution method. Consequently, all of the strains we isolated in middle Taiwan resisted many kinds of antibiotics. Furthermore, there were more than 70% strains which can resist antibiotics but only 5 kinds of antibiotics reached sensitivity over 50%, they were Colistin (93.66%), Ceftiofur (78.43%), Florfenicol (69.06%), Norfloxacin (62.5%), and Ofloxacin (50%). In addition, we chose two broiler farms that their chicks came from single breeder farm. We collected litters, waters, and cloacae swabs from these farms which were isolated 53 E. coli strains. To analyze the epidemiology of E. coli, we used Plasmid Profiling and Random Amplified Polymorphic DNA (RAPD) methods. As a result, we found that the conditions of environment and equipment closely related to the distribution of avian colibacillosis. Because the antiseptics did not use seriously, the prevalence of antibiotics-resistance strains was found at high level. The difference between antibiotics、avian species、farms and areas could induce different prevalence of antibiotics-resistance strains and infected models. We found that different strains isolated from a case had similar antibiotics resistance pattern and plasmid profiles. To sum up, by monitoring the sensitivity of antibiotics and finding out the antibiotics-resistance models, we could dwindle the transmission of E. coli.
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