| Summary: | 碩士 === 國立中興大學 === 獸醫學系 === 93 === Electric and electromagnetic fields have already been applied for therapy of bone disease at present because of promoting proliferation of osteocytes and osteoblasts. Some studies also suggested that these treatments can promote axon regeneration in injuried peripheral and central nervous system. However, how these applications effects on stem cells are unknown. Therefore, we choose bone marrow stromal cells (BMSCs) which were separated from bone marrow cells as a model of the multipotential stem cell. There are many reports claimed that BMSCs could not only differentiate into other mesenchymal cells but also trans-differentiate into non-mesenchymal cells, such as neurons, alveolar epithelial cells and thymus cells. In the present studies, we have investigated the effects of direct current (DC) electric fields on cellular proliferation, cellular viability, and signal transduction of BMSCs for different periods in vitro. By using immunochemical staining method, MTS assay and flow cytometry, we found that cell numbers of BMSC exposed to 24V/3cm or 35V/3cm DC electric fields for 48 h were significantly higher than those of controls. In order to investigate intracellular signal transduction, we used inhibitors which correlate with cell proliferation. This result indicates that treatment with either Genistein (protein tyrosine kinase inhibitor) or U0126 (MEK inhibitor) blocks the effect of DC electric fields. Then we detected intracellular phosphor-ERK 1/2 by SDS-PAGE and Weston blot. The result of western blot shows that phospho-ERK 1/2 raised from 5-7 min after DC electric fields treated. It means that DC electric fields seem to promote proliferation of BMSCs dependence on MEK/ERK pathway. We hope the application of DC electric fields combined with BMSCs transplantation in vivo to enhance cells viability and proliferation in the future.
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