Identification of Non-Tuberculous Mycobacterium in Environmental Samples by PCR-Denaturing Gradient gel Electrophoresis

• Main Authors: Wang Yu-Fen, 王郁芬
• Other Authors: Hung Chun-Hsiung
• Format: Others
• Language: zh-TW
• Published: 2005
• Online Access: http://ndltd.ncl.edu.tw/handle/77534514601538220014

碩士 === 國立中興大學 === 環境工程學系 === 93 === Nontuberculous mycobacteria (NTM) is a common microorganisms which cause diseases in immunocompromised patients. In addition, Some species may cause infections similar to tuberculosis in humans and animals.There is very limited documentation of person-to-person transmission of NTM. This aim of this study is to develop a method which can detect the NTM of the tap water of hospital. In our study, we try to combine some molecular techniques based on the 16S rRNA and the hsp gene of mycobacteria, PCR-DGGE，for identification of NTM in environmental samples. 30 pure cultures were applied in this research, but only 20 cultures showed the postive signal after PCR analysis. Five different species of NTM were identified form these 20 cultures after 16S rRNA sequencing , including M. porcinum, Mycobacterium sp., M. lacticola, M. mucogenicum, M. wolinskyi. These results demostrated the method we developed that identification nontuberculosis mycobacterium in environmental samples by PCR-DGGE has been established and more precise. In the future, we hope this efficient method could be use for definitive detection of NTM of environmental samples.