| Summary: | 碩士 === 國立中興大學 === 環境工程學系 === 93 === Abstract
According to our preoious studies, the Rhodococcus rubber N4, which cultured in the Naphthalene’s oversaturated aqueous with unsoluble Naphthalene, could be biodegraded the unsoluble Naphthalene. Some of the scientific literature has shown that R. rubber could produce biosurfactant. By monitoring the surface tension, emulsification index and cellular hydrophobicity, we could probe into the relationship between producing biosurfactant and biodegrading unsoluble Naphthalene, and to determine the four Monod kinetic parameters（Ks, Yg, μmax and kd） through the use of respirometric-two phase method.
The results indicated that in Naphthalene’s oversaturated aqueous with unsoluble Naphthalene, R. rubber N4 had shorter arrearage period and greater degrading than other pure cultures. Comparing the results of surface tension and emulsification index culturing in different mediums, we infer that producing biosurfactant was not a main factor in degrading Naphthalene by pure cultures. Getting the Monod kinetic parameters by respirometric two-phase method we found that the acclimated strains had shorter arrearage period and power in degrading Naphthalene, and specifically in R. rubber N4 after an over long period of incubaction (90 days) that we observed the microbial fingerprint graph in its Naphthalene-degrading from the variation in OUR vs. Ou graph. At high correlation between the actual Yield and getting Yg from the respirometric two-phase method（Yg＝1.25Yield, r2=0.95）,we confirmed that the method should be a trusty and feasibility one.
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