| Summary: | 碩士 === 國立中興大學 === 園藝學系 === 93 === The study was to seek effects of activated charcoal on Phalaenopsis plantlet growth and components of the gaseous environment in vitro, and effects of chemicals application on plantlet quality and Rubisco enzyme activity ex vitro.
Adding different activated charcoal concentration in medium, ethylene and dioxide carbon content in flask increased accompanied with activated charcoal concentration. Methane can also be found in the flask. There had 1.32 % methane in control at 60 days after subculture. Activated charcoal can increase leaf length to 28.7 mm and width to 15.4 mm, respectively. Root number also increased to 6.2 and root activity gave 4.04 ODA480/g F.W, but it inhibited root elongation.
Using chemicals treatment to plantlet when plantlet ex vitro. Thiamine irrigated would promote plantlet growth more than thiamine dipped. In addition, the finest performance for leaf length and root number which gave 17.89 % and 4 % were found in thiamine 1 g/l, respectively. Total soluble sugar content was increase, too. 25 mg/l ZnSO4 promoted plantlet growth and chlorophyll content, but toxic phenomenon appeared when concentration excess 50 mg/l. 200 mg/l ZnSO4 decreased leaf length 10.54 %. The effect of glycerol on leaf and shoot would more than root, 0.5 % glycerol can increase leaf length、leaf width and leaf fresh weight, but the effect of root growth were not significantly. 25 g/l sucrose solution accelerated leaf expand, leaf length and leaf width increased 45.98 % and 31.34 % that compared with control after 15 days. However, plantlet growths were restrained because sucrose accumulation, sometimes, plantlet could even appear leaf withered or root died.
Synergism between IBA and thiamine for plantlet growth were studied. Using 1 g/l IBA alone promoted root number and root fresh weight. 2 g/l IBA made plantlet toxic, exhibited root length decrease, wilt or died. 1 g/l IBA × 1 g/l thiamine promoted leaf length, root number and root length significantly. Root activity gave 4.15 ODA480/g F.W.
Comparing effects of chemicals for Rubisco enzyme activity of plantlet ex vitro. Rubisco enzyme activity of control plantlet gave 0.38 n mol RuBp carboxylase mg protein-1 min-1. 25 mg/l ZnSO4 promoted Rubisco enzyme activity which gave 0.69 n mol RuBp carboxylase mg protein-1 min-1, but ZnSO4 50 mg/l could cause restrainer. Rubisco enzyme activity could even give 0.22 n mol RuBp carboxylase mg protein-1 min-1 when 200 mg/l ZnSO4 used. The application of sucrose solution increased carbohydrate accumulation and result in Rubisco emzyme activity decreased. Applying 100 g/l sucrose solution treatment on plantlet for 30 days after ex vitro, Rubisco enzyme activity would gave 0.11 n mol RuBp carboxylase mg protein-1 min-1 only.
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