| Summary: | 碩士 === 國立中興大學 === 食品科學系 === 93 === This study was to explore the mutagenicities, antimutagenicities and anti-inflammatory effects of water extracts from Chinese medicinal plants, including Amomi fructus, Ampelopsis brevipedunculata (Maxim.) Trautv., Echinops grijsii, Millettia reticulata Benth, Rhinacanthus nasutus L. Kurz, Salvia plebeia R. Brown and Zizyphus jujuba Mill. The chemical compositions of water extracts from these prepared Chinese medicinal plants were also determined.
The results showed that among the chemical compositions including moisture, protein, lipid, ash, carbohydrate and polyphenol each water extracts were different. All the water extracts (0.001~1000 μg/plate) demonstrated no mutagenicitiy against Salmonella typhimurium TA98 and TA100 whether cultured with or without S9 mix. However, S. plebeia (10 μg/plate) and A. fructus (1000 μg/plate) inhibited the mutagenicities of S. typhimurium TA98 induced with 4-NQO (0.5 μg/plate) by 50% or above. E. grijsii, A. brevipedunculata, S. plebeia and M. reticulata at the concentration of 1000 μg/plate inhibited the mutagenicities of S. typhimurium TA98 induced with IQ (50 ng/plate) by 50% or above. E. grijsii, R. nasutus, S. plebeia and A. fructus at the concentration of 1000 μg/plate inhibited the mutagenicities of S. typhimurium TA100 induced with 4-NQO (1 μg/plate) by 50% or above. E. grijsii, A. brevipedunculata, S. plebeia and M. reticulata at the concentration of 1000 μg/plate inhibited the mutagenicities of S. typhimurium TA100 induced with IQ (1 μg/plate) by 50% or above.
To investigate anti-inflammatory effects of these water extracts from Chinese medicinal plants, pro-inflammatory cytokines, such as IL-1β, IL-6 and TNF-α, and anti-inflammatory cytokines, such as IL-10, secreted by peritoneal macrophages in vitro and in vivo from female BALB/c mice were determined. In vitro study, E. grijsii, R. nasutus, A. brevipedunculata, S. plebeia, Z. jujuba, A. fructus and M. reticulate at the concentrations of 500, 500, 250, 250, 500, 250 and 50 µg/mL, respectively, inhibited the inflammatory responses of peritoneal macrophages stimulated by lipopolysaccharide (LPS). In 28 days consecutive feeding study with female BALB/c mice, it was shown that E. grijsii, R. nasutus, A. brevipedunculata, S. plebeia, Z. jujuba, A. fructus and M. reticulate at the doses of 0.13, 0.08, 0.11, 0.07, 0.13, 0.04 and 0.06 g/kg body weight, respectively, also inhibited the inflammatory responses of peritoneal macrophages stimulated by lipopolysaccharide (LPS). The results showed that the seven Chinese medicinal plants extracts inhibited pro-inflammatory cytokines production (IL-1β, IL-6 and TNF-α) of peritoneal macrophages stimulated by lipopolysaccharide (LPS), while they increased anti-inflammatory cytokines IL-10 production. These results suggested that E. grijsii, R. nasutus, A. brevipedunculata, S. plebeia, Z. jujuba, A. fructus and M. reticulate might repress the inflammatory response induced by LPS in vitro and in vivo and demonstrate anti-inflammation potentials. These results also suggested that the water extracts from the seven medicinal plants might prevent and decelerate the injury during an inflammation status.
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