Genetic analysis of Bactrocera dorsalis(Hendel) population in taiwan using microsatellite markers

• Main Authors: Li-ching Guan, 管力慶
• Other Authors: Shaw-Yhi Hwang
• Format: Others
• Language: en_US
• Published: 2005
• Online Access: http://ndltd.ncl.edu.tw/handle/67994385652421848774

碩士 === 國立中興大學 === 昆蟲學系 === 93 === To organize an integrity monitor system on Bactrocera dorsalis (Hendel), microsatellite loci provide an ideal for population genetics study. The main goal of this study is using microsatellite loci as molecular markers to survey the population structure of B. dorsalis in Taiwan. Six hundred and thirty regional samples were collected during 2003 to 2004 from 8 different areas of Taiwan. They are named Northern, North-Eastern, Central, Southern, Eastern, Penhu, Kinmen and Laboratory population. In this assay, genomic DNA was prepared from B. dorsalis by using chelex 100 quick extraction first and subsequently analyzed by specific primers of MS3, MS4, MS5 and MS6 loci. In the following PCR reaction, mixed primers were used to amplify four loci at the meantime. Allele sizes of PCR products were determined by LI-COR sequencer with IR2 700 and IR2 800 labeled primers at final step. This improvement makes data collection more efficient and clear than previous protocol. Using GENEPOP program, the values of observed heterozygosity of different population were between 0.087 and 0.805, and the expected heterozygosity between 0.258 and 0.874. Most data indicate that the observed heterozygosity values are lower than expected, and show significant deviations from Hardy-Weinberg expectation (p<0.05). From the positive Fis value (inbreeding coefficient), the inbreed effect in each population has been expected. In addition, based on Fst, DA value and phylogenic tree, the B. dorsalis population in Taiwan can be divided into the following 4 groups based on their genetic characterization: 1) North subpopulation, 2) North-Eastern subpopulation, 3) Central subpopulation, and 4) South subpopulation that including samples from East, Penhu and Kinmen. From above modification and experimental results, the microsatellite analysis has been proven to be a sensitive and accurate way to perform B. dorsalis population studies in Taiwan.