Functional Analysis of GIGANTEA(GI) and Its Interacting Proteins in Regulating Flowering Time and Leaf Senescence in Arabidopsis thaliana

• Main Authors: Ya-Chun Li, 黎雅君
• Other Authors: Chang-Hsien Yang
• Format: Others
• Language: zh-TW
• Published: 2005
• Online Access: http://ndltd.ncl.edu.tw/handle/59569180449887546153

碩士 === 國立中興大學 === 生物科技學研究所 === 93 === Mutation in GI caused delayed flowering, high tolerance to paraquat -induced oxidative stress, high resistance to cold stress and delayed leaf senescence. These results reveal that GI should play multiple roles during plant development. To further investigate the different mechanisms regulated by GI, yeast-two hybrid was performed to identify its interacting proteins. Two proteins encoded are a chlorophyll A/B binding protein 4 precursor and an unknown functional gene (At3g51140) which showed strong interaction with GI protein. Further analysis identified an ankyrin repeat family protein (AFT) that interacted with chlorophyll A/B binding protein 4 precursor. A vesicle-associated membrane protein, NAC2 and a stress-inducible protein (At1g62740) that interacted with the unknown functional protein (At3g51140). Functional analyses of genes encoding those proteins are in progress. Furthermore, to investigate the different portions of the GI protein responsible for various function, constructs containing different truncated forms of GI protein were generated and transformed into wild-type and gi mutants for functional analysis. Further analyses of the transgenic plants transformed with these truncated forms of GI protein is in progress. The results obtained should lead a deeper understanding of the function of GI gene in regulating plant development.