Supercritical Fluids Extraction of 5,7-dihydroxy-2-4-hydroxyphenoxy-6-methoxy-chromen-4-hydroxyphenoxy fromfrom Artemisia Capillaris T. and the Growth Inhibition of Liver Cancer cells

• Main Authors: Chih-Chiang Yang, 楊志強
• Other Authors: Chieh-ming J. Chang
• Format: Others
• Language: zh-TW
• Published: 2005
• Online Access: http://ndltd.ncl.edu.tw/handle/37517663383491488932

碩士 === 國立中興大學 === 化學工程學系 === 93 === This study examined the effect of Soxhlet solvent and Supercritical CO2 (SC-CO2) extracts of Artemisia capillaris T. (AC) and Artemisia annua L. (AA) on the growth inhibition of rat hepatic stellate cells (HSC) and two human hepatoma cells (Hep-G2 and HUH7). Most of extracts of Artemisia annua L and Artemisia capillaris T. present growth inhibitions on the HSC and on the Hep-G2 and HUH7 by a varied degree. Concentration ranged from 0.01 μg/ml to 70 μg/ml for HSC cells, concentration ranged from 15 μg/ml to 400 μg/ml for hepatoma cells. Further cancer cell treatments using three pure compounds revealed that capillarisin is relatively a significant growth inhibitor for HSC cells and for two human hepatoma cells. A two factor centercomposite scheme of Response Surface Methodology was then designed for supercritical CO2 added with ethylacetate extraction at 50℃. Pressure ranged from 2000 psig to 3000 psig and co-solvent ranged from 5 wt% to 10 wt%. The AC extracted of supercritical carbone dioxide added with 7.5 wt% ethyl acetate at 2500 psig and 50℃results in the hightest purity (39.52 wt%) of capillarisin and the best growth inhibition of two hepatoma cells. AC sample’s IC50 values of growth inhibition of Hep-G2 and HUH7 cells are 72 ppm and 40 ppm, respectively, inuestrgated ender concentrations ranged from 15 μg/ml to 120 μg/ml.