Hot Pressurized Water Extraction of Syringin from Acanthopanax Senticosus and Immune Activation of Rat Blood Macrophages

• Main Authors: Chia-Shin Lin, 林家興
• Other Authors: Chiehming J. Chang
• Format: Others
• Language: zh-TW
• Published: 2005
• Online Access: http://ndltd.ncl.edu.tw/handle/94854767589981142051

碩士 === 國立中興大學 === 化學工程學系 === 93 === This study individually investigates the influence of Syringin and the hot pressurized water (HPW) extracts of Acanthopanax senticosus (AS) on the immune activation of rat blood macrophages. Soxhlet’s experimental data of 6-hour extraction of Taiwan AS wetted 12-hour indicated that Taiwan AS processes more Syringin (1.20 mg/g of solid) than that of China AS. The results of Response Surface Methodology (RSM) tuned by two factors of Temperature and SSR disclosed that temperature (110℃-120℃) is a significant factor on the recovery of Syringin extracted from Taiwan AS with the 96:1 of solvent to solid ratio in a period of 45 minutes. However, when temperature exceeded 140℃, both recovery and purity of Syringin started to decrease. The purified Syringin standard and the HPW extracts optimized by a two-factor and three-level RSM were then applied to rat’s immune activation (i.e. Macrophage growth rate, NO-released, and Phagocytosis) of rat blood Macrophages. Our results indicated the growth rate and Phagocytosis of Macrophages presented the maximum peak at the 16 mg/L of Syringin when cells were cultured 24, 48, and 72 hours. In the mean time, the release of NO also reached a stable value. Finally, The dosage of 16 mg/L of the HPW extract on the rat Macrophages for 72 hours exhibits a positive phagocytosis effect that correlated with the content of Syringin in the extract, which designed by the RSM operated at the 96:1 solvent to solid ratio, 50 psig, 120℃, and 45 minutes extraction.