Purification and immobilization of penicillin G acylase using multifunction membrane

• Main Authors: Cheng-Weng Huang, 黃振翁
• Other Authors: Yung-Chuan Liu
• Format: Others
• Language: zh-TW
• Published: 2004
• Online Access: http://ndltd.ncl.edu.tw/handle/25946379954999043951

碩士 === 國立中興大學 === 化學工程學系 === 93 === This research is constituted of two parts. The first part was to study the preparation of surface modification of the regenerated cellulose membrane to yield an immobilized metal affinity membrane（IMAM）, and the second part was to study the application of the IMAM to enzyme immobilization. For the preparation of IMAM, immobilized metal ion（Cu2+）was used to determine the proper conditions for enzyme covalently immobilized, and to construct a multifunctional membrane（MFM） for enzyme immobilization. In this work, the MFM was used to study the effect of penicillin G acylase（PGA）purification and immobilization. It is shown that when the iminodiacetic acid（IDA）was increased on the multifunctional membrane, the immobilized copper ions were increased, and the maximum content of copper ions adsorption could reach 57 mmol∕disk. The results of copper ions adsorbed amount indicated that IDA and epoxy were coexist on the membrane. The results showed that when using 0.05 M IDA reacted with membrane at 24℃ for 12 h, then incubating for 120 h to immobilize PGA. The resulting immobilized membrane could be reused 38 times at 37℃ for 60 days, and even the copper ions were eluted from the membrane with ethylenediamine tetraacetic acid disodium salt dihydrate （EDTA）, still 80% residual activity could be maintained.