Synthesis and characterization of the superparamagnetic iron oxide nanoparticles surface-modified with poly(ethylene glycol) specifically for folate receptor

• Main Authors: Yu-Chin Huang, 黃有進
• Other Authors: Yun-Ming Wang
• Format: Others
• Language: zh-TW
• Published: 2005
• Online Access: http://ndltd.ncl.edu.tw/handle/19075153269557731438

碩士 === 高雄醫學大學 === 醫藥暨應用化學系碩士班 === 93 === Superparamagnetic iron oxide nanoparticles were surface-modified with poly(ethylene glycol) (PEG) and folic acid, to improve their dispersion and target to specific cells. The success of the synthesis of the SPIO-PEG(400)-FA, SPIO-PEG(1000)-FA and SPIO- PEG(2000)-FA were confirmed by Fourier transform infrared spectroscopy (FT-IR) and UV-Visible spectrophotometer (UV-Vis). On the other hand, TEM analysis showed the well-dispersed nanoparticles after SPIO were coated with PEG and folic acid. The surface-modified with nanoparticles, which were detected by spin-lattice relaxation time（T1） and spin-spin relaxation time（T2） with 20 MHz relaxometer, demonstrated that the nanoparticles had highly relaxivity. Moreover, the nanoparticles, SPIO-PEG(400)-FA, SPIO-PEG(1000)-FA and SPIO-PEG(2000)-FA, were targeted to the human nasopharyngeal epidermoid carcinoma（KB）cells which could be proved by flow cytometric analyzer experiment. Therefore, they could be used to detect the over-expression folate-receptor cells, and have the potential to be used as the contrast agents for magnetic resonance imaging（MRI）.