| Summary: | 博士 === 高雄醫學大學 === 醫學研究所博士班 === 93 === Ruyi-Jinhuang Gao is an ointment prepared from Ruyi-Jinhuang San,
a traditional formula of powder type Chinese Medicine, usually used in
the anti-inflammatory analgesic care in China. The present studies were
aimed 1) to detect the anti-inflammatory signaling of Ruyi-Jinhuang Gao
exposed by an experimental model of lipopolysaccharides (LPS)-induced
pro-inflammatory expression of inducible nitric oxide synthase (iNOS)
and prostaglandin endoperoxide synthase (PGH synthase-2; COX-2).
LPS-induced activation of iNOS and COX-2 has been recognized to
increase cytokines and nitric oxide (NO), which of them play the
predominant roles in inflammation. In RAW264.7 cells, Ruyi-Jinhuang
Gao concentration-dependently inhibited LPS-induced iNOS and COX-2
expression. However, the herbal components of Ruyi-Jinhuang Gao
displayed different spectrum of results, including increase and decrease of
both protein expressions. Among them, inhibition by Curcuma Zedorarica
Rhizoma (Cu), Atractylodis Lancea Rhizoma (At), and Glycyrrhizae
Radix (Gl) are more potent than by others on iNOS and COX-2 inhibition
at 15 hours. This variety of expression enhanced the possibility of
formulation rearrangement. 2) Related to cytotoxicity or cell viability,
Ruyi-Jinhuang Gao showed various degree of cellular survival rate with
concentration-dependent after 6 and 15 hours of macrophage cell line
incubation. These herbal components analysis revealed that inhibitions by
Cu and At may be mainly related to their cytotoxicity. And, Glycyrrhizae
Radix (Gl) showed less cytotoxicity than Cu and At. Presumably, GI is
the most potent component of Ruyi-Jinhuang-Gao in inhibition of
LPS-induced iNOS and COX-2 expression. 3) Concerning about
Sensitization, Ruyi-Jinhuang Gao showed there was inhibition of
pro-inflammatory cytokines in compared with the LPS-induced tumor
necrosis factor-α (TNF-α)�w�z croton oil-induced cytokine formation of
TNF-α and interleukin-4 (IL-4). It indicates that Ruyi-Jinhuang Gao may
not significantly sensitize an immunologic response during the treatment
of non-wounded inflammation on the body surface.
Clinically, Ruyi-Jinhuang Patch, a patch preparation of
Ruyi-Jinhuang Gao, has proven significantly to relief cutaneous
inflammation-associated somatic pain with its benefit of less sensitization.
The pharmacologic activities of Ruyi-Jinhuang Gao may be mainly due to
blockade of iNOS and COX-2 with partial cytotoxicity.
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