| Summary: | 碩士 === 高雄醫學大學 === 醫學研究所碩士班 === 93 === Abstract
San-Ou-Sya-Sin-To(SOSST,San-Huang-Shel-Shin-Tang,San-Huang-Xie-Xin-Tang, 三黃瀉心湯), a traditional Chinese medicine, has been used for treatment a variety of ailments including hypertension, constipation and gastric ulcer. The ingredient crude drugs have been reported to possess anti-inflammatory, anti-cancer or anti-viral activities. The aim of this study was to investigate the in vitro anti-cancer（leukemia and solid cancer cells）and anti-virus（respiratory syncytial virus,RSV; coxsakie virus B1 , CVB1 and herpes simplex virus type-1, HSV-1）activities by XTT reduction method.
The results of the cytotoxic activities by two crude extracts from SOSST showed that were not different between two leukemia cell lines. In anti-k562 leukemia cells, the methanolic extracts of Coptis chinensis and Rheum officinale were more active than aqueous extracts. The cytotoxic results against human solid cancer cell lines by SOSST and related crude drugs exhibited that colon adenocarcinoma (HT-29) cells was more sensitive than lung carcinoma (A549) cells.
The results of the antiviral activities, the methanolic extract from SOSST possessed to inhibit infection of RSV, CVB1 and HSV-1, the 50% effective concentration (EC50) were 74.3±0.1, 4.6±0.3 and 35.6±0.7μg/ml, respectively. In related crude drugs, the methanolic extract from R. officinale expressed potent activity against RSV infection with the EC50 value of 9.8±1.1μg/ml. The antiviral activity (EC50) against CVB1 infection of the methanolic extracts from C. chinensis and S. baicalensis were 6.9±0.1及11.6±0.2μg/ml, respectively. The inhibition (EC50) of HSV-1 infection by R. officinale showed that aqueous extract was 35.3±1.3μg/ml and methanolic extract was 20.5±0.7μg/ml.
The results of the time-of-addition studies showed that SOSST could prevent and inhibit RSV infection. The aqueous extract from S. baicalensis prevented the infection of RSV, whereas the aqueous and methanolic extracts from R. officinale could prevent RSV infection and inhibit the replication after infection. The methanolic extracts from SOSST and R. officinale could inhibit the viral attachment, penetration, and syncytial formation induced by RSV, which confirmed the prophylactic activity against RSV infection and inhibited the virus replication after infection. The R. officinale-related pure compounds, chrysophanol, emodin and rhein, could prevent RSV virus infection, whereas sennoside A inhibited virus replication after RSV infection.
In conclusion, this study showed that the aqueous and methanolic extracts from SOSST and its ingredients and R. officinale-related pure compounds (chrysophanol, emodin, sennoside A and rhein) possessed anti-virus activities, suggesting the potential use of these drugs for prevention and treatment of viral infections. Further investigation to develop new antiviral drugs from these Chinese medicines and its pure compounds are warranted.
|
|---|
