IL-4 and IFN-γ in Chronic Periodontitis

• Main Authors: Chia-Hsin Ku, 古家欣
• Other Authors: Chi-Cheng Tsai
• Format: Others
• Language: zh-TW
• Published: 2005
• Online Access: http://ndltd.ncl.edu.tw/handle/52596252005955341400

碩士 === 高雄醫學大學 === 牙醫學研究所碩士班 === 93 === Recent studies have shown that cytokines, especially those produced by T cells, are pivotal to the immune response of chronic periodontitis and may be used as markers in diagnosis. The aim of present study was to determine the level of interleukin (IL)-4 and interferon (IFN)-γ in gingival crevicular fluid (GCF) of individuals with moderate and advanced chronic periodontitis and to study their association with clinical periodontal parameters, smoking, and the response to non-surgical periodontal treatment. A total of 70 sites of 17 patients with chronic periodontitis were included in the study. GCF was collected by means of Periopaper® at baseline and one month after non-surgical periodontal treatment. The contents of IL-4 and IFN-γwere measured in 140 samples by use of commercially available sandwich enzyme-linked immunosorbent assays produced by R&D company. In sites without bleeding on probing (BOP), the level of IL-4 was significantly higher as compared to sites with BOP before non-surgical periodontal treatment. In sites with probing depth (PD)<6 and sites with probing attachment level (PAL)<6, the level of IL-4 was significantly higher as compared to sites with PD≧6 and PAL≧6 before non-surgical periodontal treatment in smokers. Furthermore, in sites with PD≧6 and PAL≧6, the level of IL-4 in nonsmokers was significantly higher than those in smokers before non-surgical periodontal treatment. After non-surgical periodontal therapy, the clinical periodontal parameters was significantly reduced, while the total amount of crevicular IFN-γwas significantly lower and the crevicular IL-4/IFN-γratio was significantly higher compared to those before non-surgical periodontal therapy. The response to non-surgical periodontal therapy in smoking population was similar to that in non-smoking population. The present data suggest that IFN-γ,the Th1 cytokine may be predominant in diseased sites, whereas IL-4, the Th2 cytokine may reduces local inflammation. The high ratio of IL-4/IFN-γmay be related to reduced clinical periodontal parameters. The crevicular IL-4 seemed to be suppressed in smoking population.