Studies on the Chemical Constituents and Cytotoxicity of Artabotrys uncinatus (Lam.) Merr.

• Main Authors: Hsin-Yu Wang, 王心榆
• Other Authors: Yang-Chang Wu
• Format: Others
• Language: zh-TW
• Published: 2005
• Online Access: http://ndltd.ncl.edu.tw/handle/78516951897880179147

碩士 === 高雄醫學大學 === 天然藥物研究所碩士班 === 93 === Artabotrys uncinatus (Lam.) Merr. (Annonaceae) is a landscape plant (known as Eagle’s Claw) and an excellent resource of fragrant flowers. In this study, the stems of this plant were collected and extracted by MeOH. In DPPH antioxidant assay, it showed that the MeOH extract was active. The extract was then partitioned between H2O-MeOH/n-Hexane, H2O-MeOH/EtOAc to give three layers (n-Hexane, EtOAc, MeOH/H2O). Chromatographic separation of the n-Hexane and EtOAc layers yielded 33 compounds, including 7 sesquiterpenes: clovan-2β,9α-diol (1), caryolane-1,9β-diol (2), spathulenol (3), β-caryophyllene- 8R,9R-oxide (4), (-)-ent-4β-hydroxy-10α-methoxyaromadendrane (5), 4β-hydroxy-10α-methoxyaromadendrane (6), and 1-methoxy-9-caryolanol (7), 2 triterpenes: 24-methylene-lanosta-7,9(11)-dien-3β-ol (8) and 24-methylenelanosta-7,9(11)-diene-3-one (9)*, 6 flavonoids: artabotryside A (10), artabotryside B (11), apigenin (12), luteolin (13), 5-hydroxy-7,4’-dimethoxyflavone (14), and catechin (15), 7 alkaloids: liriodenine (16), atherospermidine (17), artacinatine (18), aisimilobine (19), artavenustine (20), 4,5-dioxoartacinatine (21)*, and N-P-coumaroyltyramine (22), 1 lignan: (+)-syringaresinol (23), 2 butyrolactones: (2R,3R)-3-hydroxyl-2-methylbutyrolactone (24) and tetrahydrofuran-4-methylidene-3-ol (25), 1 diterpene : phytol (26), 1 glyceryl ester : phosphoric acid mono-(1-hydroxy-3- palmitoyloxypropyl) ester (27), 6 steroids: (24R)-stigmasta-5-en- 3β,7α-diol (28), (22E,24S)-stigmasta-5,22-dien-3β,7α- diol (29), β-sitostery-3-O-β-D-glucoside (30), stigmasteryl-3-O-β-D- glucoside (31), β-stigmasterol (32), and β-sitosterol (33). Besides, compounds 34 - 48 were identified by GC-MS. The structures of these compounds were identified by spectral and mass data. Among them, 9 & 21 ars new compounds. 1-3、5-7、14-15、22、25-29 are isolated for the first time from genus Artabotrys. In addition, compounds 13, 16, 17, 23 had different cytotoxicity in MTT assay. Compounds 10, 11, 13, 15 showed different anti-oxidant ability in DPPH , SOD, and Hydroxy radical scavenging assays.