Gallomyricitrin from Syzygium samarangense inhibits cell proliferation in human peripheral blood mononuclear cells induced by phytohemagglutinin

• Main Authors: Yuh-Min Kuo, 郭裕民
• Other Authors: Yuh-Chi Kuo
• Format: Others
• Language: zh-TW
• Published: 2005
• Online Access: http://ndltd.ncl.edu.tw/handle/71913324855016750684

碩士 === 輔仁大學 === 生命科學系碩士班 === 93 === Syzygium samarangense is used to relieve inflammation and fever in Chinese medicine. The myricetin-3-O-(2”-O-galloyl)-α- rhamnopyranoside (gallomyricitrin; C28H24O16; M.W. 616) from the leaf of S. samarangense, inhibited proliferation of peripheral blood mononuclear cells (PBMCs) induced by phytohemagglutinin (PHA) . To realize its action mechanism, effects of gallomyricitrin on cell proliferation, cytokine genes expression, cell cycle progression, and signal transduction in PBMCs stimulated by PHA were investigated. The results indicated: (1) Gallomyricitrin inhibited PBMCs proliferation induced by PHA with no cytotoxicity; (2) The gene expression and protein production of cytokines, including interferon- (IFN-), tumor necrosis factor- (TNF-), interleukin-2 (IL-2), IL-4, and IL-10, was suppressed by gallomyricitrin in the concentration-dependent manner; (3) Gallomyricitrin blocked the cell cycle progression in PBMCs activated with PHA at the G0/G1 phase; (4) The proteins production and genes expression of cyclin D3 and cyclin E were not affected by gallomyricitrin; (5) The genes expression of both cyclin A and cyclin B were interrupted by gallomyricitrin; (6) Gallomyricitrin reduced the level of phosphorylated Akt in PBMCs which is stimulated by PHA; and (7) The phosphorylation of extracellular receptor-activated kinase (ERK) in PBMCs was decreased by gallomyricitrin. According to these results, the inhibitory effect of gallomyricitrin on PBMCs proliferation induced by PHA may be through reduction of NF-AT activation, decrease in ERK phosphorylation, inhibition of cytokines and cyclins production, and finally arresting cell cycle progression at G0/G1 phase.