The study of a Ling Zhi protein, LZ-8, on anti-inflammatory responses

• Main Authors: Huang Min Yu, 黃敏瑜
• Other Authors: Chou Shiu-Huey
• Format: Others
• Language: zh-TW
• Published: 2005
• Online Access: http://ndltd.ncl.edu.tw/handle/91216961824198020342

碩士 === 輔仁大學 === 生命科學系碩士班 === 93 === Abstract Ling Zhi-8 (LZ-8) is a glycoprotein purified from the mycelial extracts of Ganoderma lucidum and has immunodulatory capacities. It has been reported that LZ-8 exhibited mitogenic activity on mouse splenocytes and human peripheral blood mononuclear cells in vitro and immunosuppressive in vivo by reducing HBsAg-specific antibody production, by preventing the incidence of autoimmune diabetes in non-obese diabetic mice, and by delaying the rejection process of allogeneic islets transplantation. Accumulating data recently indicated that the incidences of many diseases, such as autoimmune disease, sepsis, and atherosclerosis, are related with the occurrence of persistence inflammatory responses. Therefore, the development of a novel anti- inflammatory drugs is one of the major goals on biomedical research. The purpose of this study is estimated the anti-inflammatory effects on LZ-8 protein. LZ-8 proteins were produced used a baker yeast (Saccharomyces cerevisiae) expression system. The concentration and bioactivity were determined before used in the experiment. Both macrophage cell line (RAW 264.7) and peritoneal excudated cell (PEC) were first used to evaluate the anti-inflammatory effect in vitro, and a LPS-induced inflammatory mouse model was further used to estimate the anti-inflammation in vivo. Results indicated that LZ-8 suppressed the nitric oxide (NO) and interleukin-6 (IL-6) production from RAW 264.7 cells after LPS and IFN-γstimulation. With Western-blot analysis, LZ-8 also inhibited the protein production of iNOS, a inducible nitric oxide synthase. Moreover, LZ-8 also suppressed NO, TNF-α, and IL-6 production in mice peritoneal excudated cells with LPS and IFN-γstimulation. The results of animal study indicated that LPS-induced the increase of IL-1β, IL-6, and TNF-αin serum and PEC were attenuated in mice which received LZ-8 injection. As well, LZ-8 decreased the production of IL-10 in PEC. In addition, LZ-8 administration could enhance the mitogenic activities of T cells in spleen and thymus of healthy mice, however, LZ-8 did not affect the change of immune activities in spleen and thymus of LPS-injected mice. In summary, LZ-8 from Ling Zhi posses the anti-inflammatory effects.