Pulsed-field gel electrophoresis analysis, toxin types and plasmid profile for Methicillin resistant Staphylococcus aureus strains isolated in Taiwan from food-poisoning cases and clinical samples between 1998-2003

• Main Authors: Chieh-hsien Lai, 賴潔賢
• Other Authors: Chi-Yue Chang
• Format: Others
• Language: zh-TW
• Published: 2005
• Online Access: http://ndltd.ncl.edu.tw/handle/54220291423056084969

碩士 === 大葉大學 === 生物產業科技學系 === 93 === ABSTRACT Methicillin-resistant Staphylococcus aureus (MRSA) is one of the major pathogens infected in community hospitals, and the incidence of MRSA strains is increasing and becomes a common problem among the hospitals in Taiwan recently. In this study, the polymer chain reaction (PCR) was used to detect the mecA resistant gene, and the Sma I restriction enzyme was used to digest the chromosomal DNA of MRSA. In addition, the plasmid profile and the pulsed field gel electrophoresis (PFGE) analysis were used to investigate the distribution of the toxin types for MRSA strains from Veterinary General Hospital (VGH) in Taichung and Center for Disease Control, Taiwan, ROC. (TCDC), and trace the MRSA infection in the hospitals. The PCR results showed that 45 (59.21 %) clinical strains from VGH and 23 (16.3 %) human S. aureus strains from food-poisoning cases provided by TCDC possessed mecA resistant gene. The ratio of MRSA strains from hospitals was higher than that from food-poisoning cases. As for the distribution for enterotoxin types, it was found that the major enterotoxin types for the strains from hospitals and food-poisoning cases were the enterotoxin A and B, respectively. From the results of the plasmid profiles for MRSA strains, totally 29 distinct plasmid types were found. Of them, no co-shared types were found in the isolates from hospitals and food-poisoning cases. The PFGE results showed that 18 PFGE types were found in the 68 MRSA strains from hospitals and food-poisoning cases in 2003, and the major types for the strains from hospitals were different from those for the strains from food-poisoning cases. In addition, the clinical strains isolated between 1998 and 2003 were found high similarity in PFGE type. The results of this study reveal that the MRSA with same PFGE type still prevail in hospitals.