Determination of Kanamycin A in Human Plasma by Capillary Electrophoresis with Laser-induced Fluorescenc Detection

• Main Authors: Yung-Chi Wang, 王永吉
• Other Authors: Yu-Chen Chang
• Format: Others
• Language: zh-TW
• Published: 2005
• Online Access: http://ndltd.ncl.edu.tw/handle/znze2q

碩士 === 朝陽科技大學 === 應用化學系碩士班 === 93 === Kanamycin A, aminoglycoside antibiotic and the second-line antituberculosis drug, is used widely for curing the infections that caused by gram-negative bacilli. and gram-positive bacilli. Because of the nephrotoxicity and ototoxicity of Kanamycin A, it is necessary to develop a rapid and sensitive method to determine the concentration in biological fluids. A capillary electrophoresis with laser-induced fluorescence detection method for the determination of kanamycin A has been developed. 6-Carboxyfluorescein succinimidyl ester (CFSE) was used for precolumn derivatization of the non-fluorescent drug. Linearity over three orders of magnitudes was generally obtained. The limit of detection for kanamycin A in aqueous solution was calculated to be 9.1 nM. The relative standard deviation of migration times and peak areas were 0.9% and 9.2% respectively. Combining with a simple cleanup procedure, this method was successfully applied to the determination of kanamycin A in human plasma. The ratio of peak area of kanamycin A / baclofen versus kanamycin’s A molar concentration plot showed a good linearity wite a correlation coefficient (r) of 0.998, and the limit of detection of kanamycin A in plasma was about 3.9 nM. The with-day and day-to-day reproducibilities of kanamycin A in plasma were less than 11.6%.