| Summary: | 碩士 === 中台醫護技術學院 === 醫學工程暨材料研究所 === 94 === This study presents a novel design for a genipin-crosslinked chitosan (GC) film incorporated into soybean protein fabric (GCS) for wound-dressing applications. Genipin, a naturally occurring cross-linking agent, was utilized to crosslink chitosan and form a wound-dressing film. Next, the genipin-chitosan mixtures were incorporated into the soybean protein non-woven fabric to generate a novel wound dressing.
This study also investigated the in vitro characteristics of the GC film and GCS dressing. Furthermore, this study utilized an in vivo rat model to investigate wounding healing using the GCS dressing. Experimental results showed that the degree of cross-linking and degradation rate of the GC film can be controlled by varying the genipin concentration.
Additionally, the concentration of genipin should exceed 0.025 wt% of the chitosan-based material if complete cross-linking reactions between genipin and chitosan molecules are required. Contact angle analysis showed that the GC film was not highly hydrophilic; therefore, the GC layers were not entangled with the
soybean protein non-woven fabric, forming an easily stripped interface layer between them.
Substances released from soaked GC film were analyzed with an ultraviolet visible light spectrophotometer. In addition, the cell culture was to determine whether or not the released substances from GC film and bletillae tuber extraction could facilitate the growth of fibroblastic cells.
Cytotoxic testing revealed that 20 ppm of the genipin in the culture medium served as the level over which cytotoxicity to fibroblastic could be produced. In addition, we found that chitosan and bletillae tuber extraction (60 ppm optimal concentration) calcium continuously were released from the GC film in the soaking solution, which promoted differentiation and proliferation of the fibroblastic.
Furthermore, this new wound dressing exhibited good mechanical properties and provided an adequate level of moisture, thereby minimizing wound dehydration. In vivo histological assessment results revealed that epithelialization and remodeling of the wound were achieved by covering the wound with the GCS dressing, indicating that it can be a potential wound dressing.
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