| Summary: | 碩士 === 中山醫學大學 === 免疫學研究所 === 93 === Type 2 diabetes mellitus (T2DM) is a metabolic disorder characterized by abnormally high blood glucose with unknown etiology. Our previous studies regarding genotypic characterization of T2DM patients revealed that individuals carrying genotypes of high interleukin-4 (IL-4) secreting ability and higher IL-4 levels were susceptible to diabetic development. Mice with higher IL-4 secreting ability would also develop T2DM more prominently than that with low IL-4 secreting ability upon stryptozotocine stimulation. Therefore, our results strongly suggested that IL-4 is involved in diabetic pathogenesis. To further investigating putative roles of IL-4 in diabetic development, protein expression patterns of 3T3-L1 adipocytes, one of the major insulin target cells, in the presence or absence of IL-4 was analyzed using proteomics techniques. Our data showed that certain proteins such as aldehyde dehydrogenase, which was involved in glucose metabolism, were regulated by IL-4. Besides, literatures showed secreted proteins from adipocytes, designated adipocytokines, play important role in glucose metabolism, lipid synthesis, and immune system. Therefore, putative differences in expression of secretory protein resulted from IL-4 treatment of 3T3-L1 fibroblasts and adipocytes were also investigated. Differential secretory protein expression patterns were observed in this study. Our data demonstrated that proteins participated in glucose and lipid metabolism expressed from adipocytes may be up-regulated and down-regulated by IL-4 treatment. These proteins may accelerate ATP synthesis, and subsequently lead to hyperlipidemia and eventually T2DM.
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