The study of the factors that influence the measurement of blood levels of superoxide and hydroxyl radical and the study of the free radical levels in healthy adults and patients

• Main Authors: Yuan Yu, 于 湲
• Other Authors: 周芬碧
• Format: Others
• Language: zh-TW
• Published: 2005
• Online Access: http://ndltd.ncl.edu.tw/handle/77141070772434807876

碩士 === 中山醫學大學 === 生化暨生物科技研究所 === 93 === A free radical is an atom, ion, or a molecule which has at least one unpaired electron. Due to a extremely short half-life, free radical is very unstable and has a high reactivity rate. In order to stabilize themself, free radicals are likely attack other molecules, such as proteins, carbohydrates, or lipids, to obtain electrons and causes a chain of free radical reaction. These chain reactions produce continous increases of new free radicals. Fortunately life forms use antioxidant defense systems (antioxidant enzymes, antioxidant) to decrease the production of free radicals in aerobic organisms, keep a balance between oxidants and antioxidants and thus protect cell from the damage caused oxidative stresses. However, when there are too many free radicals and the balance is offset, the free radicals will cause damage to cells or tissue and lead to many diseases, such as cardiovascular, respiratory and nervous defect system, ageing and cancer. This study uses Ultra-weak chemilumines- cence Analyzer to analyze the circular levels of superoxide and hydroxyl radical of healthy adults and patients with various diseases. We first studied two variables, experimental time and the anticoagulant used, that might affect the measurement of free radicals. Then, the relationships between the concentration of free radicals and the age, body condition, living habits, blood test data and diseases of subjects were examined. The result showed that these factors (experimentational time, anticoagulant ingredients or individual parameters) do not affect the levels of free radicals. However, healthy people have lower levels of free radicals than patients with three types of diseases. Therefore, it is suggested that superoxide and hydroxyl radical should not be used as an indication of disease, instead should be used as a follow-up reference to stay healthy.