Inhibitory effect of Ailanthoidol on TPA induced inflammation and tumor promotion

• Main Authors: Yea-Huey, 邱雅惠
• Other Authors: Tsui-Hwa Tseng
• Format: Others
• Language: zh-TW
• Online Access: http://ndltd.ncl.edu.tw/handle/43004121587940714686

碩士 === 中山醫學大學 === 生化暨生物科技研究所 === 93 === Ailanthoidol (AT), a membr of neolignan, has been obtained from the stem woods of Zanthoxylum ailanthoides (1,2). Evidence demonstrated that neolignan and lignan exhibit a broad range of biological activites such as antiproliferation, antivirus, antioxidant, caner preventative and anti-inflammanatory. In the preliminary study, AT showed an effective antioxidant property. Further investigations showed that the topical application of AT (0.5, 1.0 and 2.5 mM) inhibited TPA-induced the incidence of edema in mouse ear to 45, 68 and 96%, respectively. The topical application of AT inhibited TPA (5 nmol)-caused induction of epidermal ornithine decarboxylase (ODC) and myeloperoxidase (MPO) activity. The same doses of AT also reduced the formation of hydrogen peroxide in the mouse skin to an inhibition of 83, 90 and 96%, respectively. In addition, biological analysis revealed the same AT inhibited inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2) protein expression. Pre-application of AT also afforded significant as protection histopathological changes in TPA-induced hyperplasia on mouse skin. To investigate Topical application of AT with TPA three times one week for 24 weeks to mice previously Benzo[a]pyrene（B[a]P）inhibited the number tumors per mouse. Finally, our results indicate that AT can inhibit TPA-induced inflammation and tumor promotion.